3B Mitochondrial DNA Analysis Using PCR Experiment
The 3B Mitochondrial DNA Analysis Using PCR Experiment is an ideal addition for teaching or learning about DNA. Using the Nobel Prize winning technique polymerase chain reaction, students can amplify two regions of DNA from the mitochondria.
Mitochondria are ideal for genetic studies as they only inherit the maternal line and are believed to be a symbiotic relationship between prokaryotic and eukaryotic cells. Students will find it useful for genetic and forensics testing and the study of evolution as a whole.
Features of the 3B Mitochondrial DNA Analysis Using PCR Experiment
- Students can carry out a DNA extraction, followed by a PCR, then use DNA electrophoresis to analyse the results
- Allows students to amplify two regions of DNA
- Uses mitochondria for their usefulness in the areas of evolution and genetics
- Suitable for classrooms and learning environments
- Contains everything required for the experiment
- Suitable for up to 25 students
What does the 3B Mitochondrial DNA Analysis Using PCR Experiment Contain?
- Proteinase K
- PCR beads
- Control DNA and primers
- Chelating agent
- DNA ladder
- Practice gel loading solution
- Gel loading dye
- Electrophoresis buffer
- Gel stain
Timings for the Experiment
- Set up timing: 30 mins
- PCR: 2 hours
- Overnight Electrophoresis: 45 mins